Reading the abstract for this paper, 'Effects of culture conditions on in vitro bulblet induction in the medicinal plant Amana edulis (Miq.) Honda ' one gets the impression that speed of production for nedicinal purposes is key - and faster than seed, of course ...
Article
Effects of culture conditions on in vitro bulblet induction in the medicinal plant Amana edulis (Miq.) Honda
Zaibiao Zhu, Lifang Zhu, Qiaosheng Guo, Jianluo Lin, Yuanyuan Miao & Xiaohua Yang
The Journal of Horticultural Science and Biotechnology
Volume 92, 2017 - Issue 6 Pages 660-667
Published online: 11 Apr 2017
ABSTRACT
Amana edulis (Miq.) Honda is an important medicinal plant with a variety of anti-cancer properties, and it is of great importance to improve its reproductive rate through micropropagation technology to meet increasing demand. In the present study, in order to establish a rapid in vitro bulblet propagation protocol for A. edulis, an L16 (45) orthogonal design was used to investigate the effects of sucrose, 6-benzyladenine (6-BA), α-naphthaleneacetic acid (NAA), and macro-elements on bulblet induction. The results show that the sucrose concentration was the crucial factor for A. edulis bulblet initiation, followed by 6-BA and macro-elements, while NAA had the weakest effect. The optimal medium for in vitro bulblet induction was Murashige and Skoog medium supplemented with 0.1 mg·L−1 6-BA, 0.01 mg·L−1 NAA, and 100 g·L−1 or 80 g·L−1 sucrose (pH 5.

, in which A. edulis shoot clusters (without roots) were cultured at 5(±2)°C for 35 d or 60 d and then incubated at 23(±2)°C for 90 d. The entire cultivation process occurred in the dark. The present study demonstrates that this protocol can be used for the propagation of A. edulis.
Some forum members grow this plant well I wonder what their experience is with seed?
From the Forum 2016 - from Steve Garvie
