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I would separate them after one year simply because small plants transplant better.
The best time to transplant any plant is probably just before new roots develop. The problem is to know when this is. Many plants will develop new roots any time of the year. However, the mechanisms that prompt some woodlanders to develop roots in time for the spring also seems to prevent them from forming roots at other times. I think observations about the time for root development would be very valuable for all of us. Perhaps such information should be collected in a sticky thread.I am only 100% certain about Trillium grandiflorum which I have moved in various seasons. I am 90% sure about Lilium martagon both start root formation when the first flowers start to fade.