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Author Topic: Liparis loeselii, Fen Orchid  (Read 2483 times)

Frans IJsselstijn

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Liparis loeselii, Fen Orchid
« on: September 04, 2007, 10:07:48 PM »
Hello everybody

Who have experience wit invitro sowing Liparis loeselii
The seeds germinate well but didn’t grow further.
I sow on BM 1 and replay on 1/5 MS

Many thanks for the answers
Frans
« Last Edit: September 06, 2007, 01:29:07 PM by Maggi Young »
in the Netherlands

Auricular

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Re: Liparis loeselii
« Reply #1 on: September 05, 2007, 08:28:10 AM »
Hello Frans,

use 1/2 MS & they grow better!

Kind regards


Bernie

Andrew

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Re: Liparis loeselii
« Reply #2 on: September 05, 2007, 04:19:33 PM »
Did everybody else understand this ?

Frans and Bernie, would you like to expand/explain the above, agars ?
Andrew, North Cambridgeshire, England.

Brian Ellis

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Re: Liparis loeselii
« Reply #3 on: September 05, 2007, 04:23:42 PM »
No I didn't either Andrew - but then I could have been having a senior moment :-X
Brian Ellis, Brooke, Norfolk UK. altitude 30m Mintemp -8C

Carlo

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Re: Liparis loeselii
« Reply #4 on: September 05, 2007, 04:31:31 PM »
Orchid seed is minute and has no food reserves. Consequently, for success with orchid seed it is usually necessary to sow on artificial nutrient media (usually an agar with added nutrients). This is done under aseptic, laboratory conditions, or in a homemade HEPA unit like hospitals use. The BM and MS abbreviations are for different recipes of medium
Carlo A. Balistrieri
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Andrew

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Re: Liparis loeselii
« Reply #5 on: September 05, 2007, 04:42:05 PM »
The BM and MS abbreviations are for different recipes of medium.

Thank you Carlo. I guessed it was about agar, it was the abbreviations I would like to know. Also 1/2 (MS) half strength, diluted with water ?
Andrew, North Cambridgeshire, England.

 


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