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Author Topic: Asplenium scolopendrium: spore seedlings  (Read 1833 times)

Véronique Macrelle

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Asplenium scolopendrium: spore seedlings
« on: March 13, 2020, 05:52:18 AM »
I picked a leaf with sori, left it a week in an envelope to dry: the spores fell in the envelope, then I sowed on hot potting soil: prothalles germinate in 2 weeks, but grow very slowly.
 currently the prothalles are 3 months old and the blades are 5 mm wide.660641-0660643-1

Véronique Macrelle

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Re: Asplenium scolopendrium: spore seedlings
« Reply #1 on: March 21, 2020, 09:17:42 AM »
that's it, there was fertilization! baby ferns are starting to develop.

I send a mist of water a few times to wet the surfaces of the prothalles. (male gametes swim in water)

and a first little fern leaf appears! top right, it is 5 mm wide and 1 cm high.

on the magnification we see:
a crater, no doubt, the place of fertilization.
we can clearly see 3 fern shoots around.

662051-0662053-1

Anders

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Re: Asplenium scolopendrium: spore seedlings
« Reply #2 on: March 21, 2020, 11:06:07 AM »
Hi Veronique

Great and inspiring fotos. Picked a few leaves of A. rute-muraria and A. trichomanes this morning to try to germinate the spores.

Anders

Véronique Macrelle

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Re: Asplenium scolopendrium: spore seedlings
« Reply #3 on: March 21, 2020, 08:32:17 PM »
thank you Anders

this is how i installed the fern spores

Carolyn

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Re: Asplenium scolopendrium: spore seedlings
« Reply #4 on: March 21, 2020, 08:48:54 PM »
Anders,

I am not a fern expert, but I have grown a few ferns from spores. I think you will find that the spores are ripe in late summer - do correct me if I am wrong! Here are some photos of my attempts from early last September.

1.  Adiantum pedatum has made a good potful. I will let these get slightly bigger and then pot them up. They have spent the winter enclosed in a plastic bag on a shady windowsill.
2.  Sphenomeris chinensis BSWJ6108 - I am hopeful that this is what is growing. It always starts by looking like an unpromising mass of liverwort! Sown 8th Sept 2019
3.  Athyrium vidallii, sown 29th Aug 2019
Carolyn McHale
Gardening in Kirkcudbright

ruweiss

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Re: Asplenium scolopendrium: spore seedlings
« Reply #5 on: March 21, 2020, 09:05:35 PM »
Amazing to watch growing ferns from spores
Rudi Weiss,Waiblingen,southern Germany,
climate zone 8a,elevation 250 m

Véronique Macrelle

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Re: Asplenium scolopendrium: spore seedlings
« Reply #6 on: March 22, 2020, 04:55:41 AM »
Carolyn
you are more expert than me! it is successful ..
because I’m doing this for the first time.

now that i see prothalles and small leaves, i'm proud, but i didn't know if it would work ...

and transplanting, is there a method, a period?

Anders
put your leaves to dry for a few days in an envelope, you will see if spores still fall from it ... it must be like dust ...
« Last Edit: March 22, 2020, 04:59:45 AM by Véronique Macrelle »

Carolyn

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Re: Asplenium scolopendrium: spore seedlings
« Reply #7 on: March 22, 2020, 08:14:16 AM »
Veronique,
I just wait till the little plants are big enough to handle (maybe 1 - 2cm tall?) then transplant them to small pots with ordinary multi-purpose compost. I keep them wet and shady - enclosed in a plastic bag for a while. Et voila!

i have no idea whether this is the best procedure, but it seems to work!
Carolyn McHale
Gardening in Kirkcudbright

Anders

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Re: Asplenium scolopendrium: spore seedlings
« Reply #8 on: March 24, 2020, 04:57:57 PM »
Carolyn, you are right. Only empty sporangia on A. trichomanes. But there were still a bit of spores left in the sporangia on A. rute-muraria  and A. scolopendrium. I wonder if they can be crossed if I mix the spores in one of the pots.

Anders
« Last Edit: March 24, 2020, 07:01:18 PM by Anders »

Carolyn

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Re: Asplenium scolopendrium: spore seedlings
« Reply #9 on: March 24, 2020, 06:49:33 PM »
Anders,
Interesting that you still found some spores - I wonder how long they remain viable? I have no idea of the answer to your query about crossing. We need a fern expert! Anyone?
Carolyn McHale
Gardening in Kirkcudbright

annew

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Re: Asplenium scolopendrium: spore seedlings
« Reply #10 on: March 24, 2020, 11:08:12 PM »
I think the usual method for hybridising ferns is to sow the spores together, then make sure the prothalli are kept at high humidity (enclosed in a polythene bag) this is to make sure that there is a film of moisture for the male gametes to swim through. If the male (antheridia) and female (archegonia) structures are studied with a hand lens or microscope it can be seen when they become ripe, then the pot can be submerged in warm water, so that the water level just rises through the prothallus mat. This makes it easier for the sperm cells to swim from one species' prothallus to that of the other species.
It is worth noting that if prothalli are very crowded, they will not produce sexual organs, so if you have sown very thickly you will need to 'patch out' small sections of the mat onto fresh sterilised compost, leaving space between them, then place again in a bag or box. This will often induce production of the sexual organs. Keep the young plants with fronds in the closed plastic bag/box for a while then when they look ready to pot on, gradually open up the bag over 2-3 weeks to get the plants ready to take the lower humidity outside.
Good luck!
MINIONS! I need more minions!
Anne Wright, Dryad Nursery, Yorkshire, England

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Maggi Young

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Re: Asplenium scolopendrium: spore seedlings
« Reply #11 on: March 25, 2020, 10:14:22 AM »
As if by  magic, one  of  our  fern experts  appears - thank you  Anne!  :-*
Margaret Young in Aberdeen, North East Scotland Zone 7 -ish!

Editor: International Rock Gardener e-magazine

annew

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Re: Asplenium scolopendrium: spore seedlings
« Reply #12 on: March 25, 2020, 10:19:54 AM »
As if by  magic, one  of  our  fern experts  appears - thank you  Anne!  :-*
The magic that is Maggi Young  ;)
MINIONS! I need more minions!
Anne Wright, Dryad Nursery, Yorkshire, England

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