Scottish Rock Garden Club Forum

Seedy Subjects! => Grow From Seed => Topic started by: Frans IJsselstijn on September 04, 2007, 10:07:48 PM

Title: Liparis loeselii, Fen Orchid
Post by: Frans IJsselstijn on September 04, 2007, 10:07:48 PM

Hello everybody

Who have experience wit invitro sowing Liparis loeselii
The seeds germinate well but didn’t grow further.
I sow on BM 1 and replay on 1/5 MS

Many thanks for the answers
Frans

Title: Re: Liparis loeselii
Post by: Auricular on September 05, 2007, 08:28:10 AM

Hello Frans,

use 1/2 MS & they grow better!

Kind regards


Bernie

Title: Re: Liparis loeselii
Post by: Andrew on September 05, 2007, 04:19:33 PM

Did everybody else understand this ?

Frans and Bernie, would you like to expand/explain the above, agars ?

Title: Re: Liparis loeselii
Post by: Brian Ellis on September 05, 2007, 04:23:42 PM

No I didn't either Andrew - but then I could have been having a senior moment :-X

Title: Re: Liparis loeselii
Post by: Carlo on September 05, 2007, 04:31:31 PM

Orchid seed is minute and has no food reserves. Consequently, for success with orchid seed it is usually necessary to sow on artificial nutrient media (usually an agar with added nutrients). This is done under aseptic, laboratory conditions, or in a homemade HEPA unit like hospitals use. The BM and MS abbreviations are for different recipes of medium

Title: Re: Liparis loeselii
Post by: Andrew on September 05, 2007, 04:42:05 PM

Quote from: Carlo on September 05, 2007, 04:31:31 PM

The BM and MS abbreviations are for different recipes of medium.

Thank you Carlo. I guessed it was about agar, it was the abbreviations I would like to know. Also 1/2 (MS) half strength, diluted with water ?