Scottish Rock Garden Club Forum

Specific Families and Genera => Pleione and Orchidaceae => Topic started by: Joakim B on January 12, 2019, 07:36:12 PM

Title: Terrestrial Orchids 2019
Post by: Joakim B on January 12, 2019, 07:36:12 PM
Did not see any local orchids while visiting Portugal only Cymbidiums and we were a bit early/ or the cold weather made the flowers late and a bit damaged

white and pink seems to be very common
Burgondy we also have a few
A yellow is a the stronger flowerer
a mustard flowered one is growing more in the open
A butter colored one
Title: Re: Terrestrial Orchids 2019
Post by: aldo on January 13, 2019, 07:56:29 PM
Calanthe cardioglossa

(http://i292.photobucket.com/albums/mm24/2111aldo/P1080156_zpsffucyizl.jpg) (http://s292.photobucket.com/user/2111aldo/media/P1080156_zpsffucyizl.jpg.html)
Title: Re: Terrestrial Orchids 2019
Post by: aldo on January 13, 2019, 07:58:14 PM
Stenorrhynchos speciosum

(http://i292.photobucket.com/albums/mm24/2111aldo/P1080149_zps3mqyebuo.jpg) (http://s292.photobucket.com/user/2111aldo/media/P1080149_zps3mqyebuo.jpg.html)
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on January 19, 2019, 09:36:05 PM
First Mediterranean Orchids in flower here:

Himantoglossum robertianum
(https://c2.staticflickr.com/8/7808/32927667018_a4cff175df_o_d.jpg)

Ophrys kotschyi
(https://c1.staticflickr.com/5/4871/32927765058_c78d74c8f5_o_d.jpg)

Ophrys tenthredinifera
(https://c2.staticflickr.com/8/7873/32927765128_9635210a67_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: aldo on January 23, 2019, 12:40:59 PM
Diuris magnifica

(http://i292.photobucket.com/albums/mm24/2111aldo/P1080174_zpskfyr3qne.jpg) (http://s292.photobucket.com/user/2111aldo/media/P1080174_zpskfyr3qne.jpg.html)
Title: Re: Terrestrial Orchids 2019
Post by: Yann on January 23, 2019, 10:18:53 PM
lovely, all those colors give some warm.
Title: Re: Terrestrial Orchids 2019
Post by: aldo on February 06, 2019, 09:58:58 PM
Thelymitra luteocilium x paludosa

1(http://i292.photobucket.com/albums/mm24/2111aldo/P1080222_zpsed8lab4z.jpg) (http://s292.photobucket.com/user/2111aldo/media/P1080222_zpsed8lab4z.jpg.html)

Thelymitra Cinderella

(http://i292.photobucket.com/albums/mm24/2111aldo/P1080227_zpsodmhlfyc.jpg) (http://s292.photobucket.com/user/2111aldo/media/P1080227_zpsodmhlfyc.jpg.html)

Thelymitra Kay Nesbitt

(https://i292.photobucket.com/albums/mm24/2111aldo/P1080226_zpshum0owgl.jpg) (https://s292.photobucket.com/user/2111aldo/media/P1080226_zpshum0owgl.jpg.html)
Title: Re: Terrestrial Orchids 2019
Post by: Yann on February 10, 2019, 05:12:20 PM
It's a slow start inside my Ophrys, Ophrys dictynnae (tenthredinifera group)
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on February 11, 2019, 08:57:59 AM
Ophrys tenthredinifera -a different form (or species?) from my earlier-flowering plant.
(https://c2.staticflickr.com/8/7823/32111076097_815805d89c_o_d.jpg)


Orchis anatolica
(https://c2.staticflickr.com/8/7907/32111076387_447c0f51e1_o_d.jpg)


Ophrys sicula (-I think this is sicula rather than phryganae but would welcome advice).
(https://c2.staticflickr.com/8/7876/32111076217_be24776f10_o_d.jpg)


Diuris orientis -one of the “Donkey orchids”.
(https://c2.staticflickr.com/8/7896/47000764362_18e0729891_o_d.jpg)

(https://c2.staticflickr.com/8/7804/47000764402_db9195b103_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on February 20, 2019, 08:51:16 AM
Ophrys speculum
(https://c2.staticflickr.com/8/7906/47135887271_274cf75358_o_d.jpg)

Ophrys helenae
(https://c2.staticflickr.com/8/7889/47135887351_2e8aa117f2_o_d.jpg)

Ophrys lutea
(https://c2.staticflickr.com/8/7897/47135887541_aae43c4390_o_d.jpg)

Ophrys bombyliflora
(https://c2.staticflickr.com/8/7806/47135887671_47c301b64b_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: Yann on February 27, 2019, 07:22:04 PM
Ophrys ferrum-equinum, i didn't feed it last year, the plant is a bit slight
Anacamptis papilionacea ssp papilionacea
Ophrys basilissa
Ophrys attica
Ophrys cretica subsp. ariadnae
Title: Re: Terrestrial Orchids 2019
Post by: Maggi Young on February 28, 2019, 02:07:32 PM
Quite extraordinary photos from Portugal - posted on Twitter  .....
Wild Orchids UK & Ireland @ukorchids

"Incredible photos from central Portugal by Pedro Jesus: Early Purple Orchids growing 2m from the ground along the trunk of a large Oak. I've never seen anything remotely like this 😮😮😮😮😮"

[attachimg=1]

[attachimg=2]

[attachimg=3]

[attachimg=4]

Isn't this  fantastic?
Title: Re: Terrestrial Orchids 2019
Post by: sjusovare on March 01, 2019, 06:38:01 AM
I wonder if that's how most of orchids evolved toward an epiphytic mode....
Title: Re: Terrestrial Orchids 2019
Post by: ian mcdonald on March 01, 2019, 01:12:02 PM
Nice pictures. Early purples grow on rock ledges in Scotland in company with alpine plants.
Title: Re: Terrestrial Orchids 2019
Post by: Maggi Young on March 01, 2019, 04:18:36 PM
I wonder if that's how most of orchids evolved toward an epiphytic mode....
Yes, I wondered that, too.
Title: Re: Terrestrial Orchids 2019
Post by: PLANTMAN1 on March 05, 2019, 08:31:39 AM
I have just been doing some symbiotic culture of Platanthera bifolia (B1) and this has grown which is different from anything else. It looks like a rhizome rather than a tuber. Has anyone seen anything like this before- its 3 cm long and 5 months old.
Title: Re: Terrestrial Orchids 2019
Post by: Neil on March 09, 2019, 08:58:12 AM
looks like it is a tuber to me, 5 months is long enough for it to start elongating, and this one seems to have just got carried away.   If it where a rhizome it would be much thinner about and you would have more than one growing out.
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on March 09, 2019, 08:47:27 PM
Ophrys ferrum-equinum
(https://farm8.staticflickr.com/7868/32347437927_430010d092_o_d.jpg)

Ophrys sphegodes
(https://farm8.staticflickr.com/7871/32347438127_b3b0b9028c_o_d.jpg)

Ophrys calocaerina
(https://farm8.staticflickr.com/7846/32347438057_4cfbbb4c35_o_d.jpg)

Ophrys lutea -a different clone from the one posted earlier.
(https://farm8.staticflickr.com/7818/32347437087_ce8e5d90f5_o_d.jpg)

Ophrys speculum
(https://farm8.staticflickr.com/7836/32347436877_7125e7da8a_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: PLANTMAN1 on March 10, 2019, 03:32:07 PM
6 years ago I bought a O. ferrum-equinum and it came up as O. cephalonica. I bought another one last year and this time its O helenae!
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on March 12, 2019, 08:56:23 PM
Anacamptis champagneuxii
(https://farm8.staticflickr.com/7892/40374861263_1453e30790_o_d.jpg)

Anacamptis morio
(https://farm8.staticflickr.com/7921/40374860973_dea0cd8e68_o_d.jpg)

Anacamptis papilionacea
(https://farm8.staticflickr.com/7909/40374861113_58c5e073e5_o_d.jpg)

Serapias cordigera
(https://farm8.staticflickr.com/7804/40374861583_2c5101b1f2_o_d.jpg)

Serapias orientalis
(https://farm8.staticflickr.com/7903/40374861423_b4b0fd97b7_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: Carolyn on March 12, 2019, 10:17:03 PM
Wonderful pics as usual, Steve!
Title: Re: Terrestrial Orchids 2019
Post by: Yann on March 31, 2019, 07:44:57 PM
Orchis anthropophora
Orchis quadripunctata x collina
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on April 04, 2019, 02:04:22 PM
Orchis anthropophora
(https://live.staticflickr.com/7810/40565401703_5dd4c6d59e_o_d.jpg)

Orchis quadripunctata
(https://live.staticflickr.com/7820/47478625632_9a472d984a_o_d.jpg)

Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on April 08, 2019, 09:19:32 AM
Caladenia (latifolia x flava) x flava -a manmade cross which mirrors the hybrid swarms of these species in the wild.
(https://live.staticflickr.com/7924/47478574722_449eaf3dfd_o_d.jpg)

Dactylorhiza romana
(https://live.staticflickr.com/7909/47478584292_eba42c7fd0_o_d.jpg)

Diuris behrii
(https://live.staticflickr.com/7831/33654698028_ea180bd55d_o_d.jpg)

Changnienia amoena -2nd year of flowering. Obtained as a young plant from a German source, it took 4 years to reach flowering size. Grown in a perlite/moler clay granules mix with a small amount of pine duff and watered with rainwater acidified by cider vinegar (7.5mls per litre). Dormant from May to September when it needs to be gently dried but never parched.

(https://live.staticflickr.com/7848/46615944365_2b0508b7f8_o_d.jpg)

(https://live.staticflickr.com/7876/46615942565_2dcde445fc_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: Erwinia on April 09, 2019, 07:58:43 AM
Beautiful pictures, Steve, in particular of the Changnienia. I tried this species only once and found out that nothing attracts slugs better than this species. It was a short trial, therefore.
Title: Serapias pollinia?
Post by: PLANTMAN1 on April 19, 2019, 06:57:11 PM
I want to cross A. laxiflora with a Serapias but have no pollen for the Serapias this year.
Has anyone any serapias pollinia they could send me and I'll send half the seeds I produce back to you?
Thanks
Title: Re: Terrestrial Orchids 2019
Post by: Gail on April 24, 2019, 10:44:09 PM
This Calanthe was an impulse buy at Aylsham garden centre this afternoon, seduced by the wonderful sweet scent.
Anyone know what species, it was just labelled Calanthe. And this small bed on the north side of my potting shed is the coolest, shadiest spot I have - do you think it is worth trying it planted out here or should I try to keep it in a pot?
Title: Re: Terrestrial Orchids 2019
Post by: GordonT on April 25, 2019, 12:08:09 PM
Gail, that Calanthe is in the perfect image of health! I think it may be Calanthe striata, (syn Calanthe sieboldii you can see more here: http://www.orchidspecies.com/calstriata.htm (http://www.orchidspecies.com/calstriata.htm)

I tried and failed with C discolor when I was living in Vancouver, so perhaps some others might be able to offer you advice on planting out or not.
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on May 03, 2019, 08:25:13 AM
Thelymitra glaucophylla
(https://live.staticflickr.com/65535/33879399698_91164b71ae_o_d.jpg)


Thelymitra rubra
(https://live.staticflickr.com/65535/33879398938_e70e687947_o_d.jpg)

(https://live.staticflickr.com/65535/33879398078_86e8728194_o_d.jpg)


Thelymitra pauciflora
(https://live.staticflickr.com/65535/33879397198_dd0528bf77_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on May 09, 2019, 09:47:54 PM
Anacamptis pyramidalis -a dark form.
(https://live.staticflickr.com/65535/32861727607_a31ef1e18c_o_d.jpg)

Anacamptis picta
(https://live.staticflickr.com/65535/32861726517_9e57c2de49_o_d.jpg)

Anacamptis laxiflora
(https://live.staticflickr.com/65535/32861725317_2ea444dc1b_o_d.jpg)

Ophrys cerastes -the last Ophrys to flower here.
(https://live.staticflickr.com/65535/47016037994_59c2d750a2_o_d.jpg)

Dactylorhiza sambucina
(https://live.staticflickr.com/65535/32861735867_ced19a5b7c_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on May 11, 2019, 11:26:52 PM
Orchis militaris
(https://live.staticflickr.com/65535/32861791797_240a152665_o_d.jpg)

Orchis tridentata
(https://live.staticflickr.com/65535/32861796347_55fce27f74_o_d.jpg)

Orchis purpurea
(https://live.staticflickr.com/65535/32861794057_8e6955e361_o_d.jpg)

Orchis mascula
(https://live.staticflickr.com/65535/32861790917_11e9003675_o_d.jpg)

Orchis ustulata
(https://live.staticflickr.com/65535/47753171192_ac3b13e1ab_o_d.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: Neil on May 12, 2019, 11:37:10 AM
Very nice Steve.
Title: Re: Terrestrial Orchids 2019
Post by: Yann on May 13, 2019, 10:31:07 PM
Nice collection
Title: Re: Terrestrial Orchids 2019
Post by: Steve Garvie on May 13, 2019, 10:41:42 PM
Many thanks Neil and Yann.

Here are some plants I am looking after for a friend.
Orchis italica -dark and pale forms.
(https://live.staticflickr.com/65535/47016063264_e64b793e0d_o_d.jpg)

(https://live.staticflickr.com/65535/47016064634_345c768b83_o_d.jpg)


Orchis italica x Orchis anthropophora two different forms:
(https://live.staticflickr.com/65535/32861723007_760fc8afe5_o_d.jpg)

(https://live.staticflickr.com/65535/32861724667_573ea2f779_o_d.jpg)


Title: Re: Terrestrial Orchids 2019
Post by: Neil on May 14, 2019, 09:45:24 AM
Don't give them back Steve!  That pale Italica is nice.
Title: Re: Terrestrial Orchids 2019
Post by: Claire Cockcroft on May 14, 2019, 05:35:03 PM
Cypripedium parviflorum, received as two small plants 7 years ago from Rimmer de Vries.  Thank you, Rimmer!
Title: Re: Terrestrial Orchids 2019
Post by: Tim Harberd on May 15, 2019, 10:00:14 AM
Hi Steve,
    Thanks, particularly, for the Orchis ustulata pic... One of my favourites.

     I'm off to the alps next week, so maybe I'll be lucky enough to renew my acquaintance with it.

Tim DH
Title: Re: Terrestrial Orchids 2019
Post by: Anders on May 17, 2019, 08:53:17 AM
Cypripedium Sabine Light and a Cypripedium parviflorum seedling, the largest and smallest Cyps in my collection. Toady, I have pollinated Sabine Light with pollen from Cyp henryi that has multiple flowers per stem. Also a picture of the last flowers on Cymbidium floribundum, which is not really a terrestrial.

Anders
Title: Re: Terrestrial Orchids 2019
Post by: sjusovare on May 20, 2019, 06:41:01 PM
Really nice picture Steve, as usual :)

On a side note, does anyone knows a source for Cyp Hank Small? I had it for years... and this spring I found a hedgehog half burried where my cyp was supposed to be...
Title: Re: Terrestrial Orchids 2019
Post by: GordonT on May 21, 2019, 02:54:15 PM
Julien, I have no idea about this vendor, but found it listed here, at a nursery in Combrit : https://www.achat-vente-palmiers.com/en/hardy-garden-orchids/1534-cypripedium-hank-small.html#.XOQAw47YrnE (https://www.achat-vente-palmiers.com/en/hardy-garden-orchids/1534-cypripedium-hank-small.html#.XOQAw47YrnE)
Title: Re: Terrestrial Orchids 2019
Post by: David Nicholson on May 31, 2019, 11:52:42 AM
Bletilla striata 'Albostriata'

[attachimg=1]

A dark form of Dactylorhiza just about buried by surrounding vegetation

[attachimg=2]
Title: Re: Terrestrial Orchids 2019
Post by: Anders on June 01, 2019, 12:42:52 PM
Epipactis mairei is one of my favourite helleborines, easy to grow and the new shoots have an intense color. E. gigantic and E. Passionata is attached for comparison.

Anders
Title: Re: Terrestrial Orchids 2019
Post by: PaulFlowers on June 21, 2019, 05:20:52 AM
Heath spotted orchid (I think)....one solitary plant at Roughcastle, Falkirk
Title: Re: Terrestrial Orchids 2019
Post by: PaulFlowers on June 21, 2019, 05:24:13 AM
Northern marsh orchids around Falkirk wheel. There are even some at Falkirk high station to cheer me up for the commute
Title: Re: Terrestrial Orchids 2019
Post by: PaulFlowers on June 21, 2019, 05:26:48 AM
Common spotted orchids - I love the variation in the patterns.
Title: Re: Terrestrial Orchids 2019
Post by: Roma on July 15, 2019, 03:21:48 PM
It must be 3 years since I last checked up on this colony of Goodyera repens, creeping ladies' tresses.  It is quite close to my house but the wood was thinned two years ago and lying branches, small logs and trenches from the heavy tree harvester made access a little difficult.  I made the effort a couple of days ago and was pleased to see there are a few patches of flowering plants.  The area they are growing in is close to the edge of the wood so they did not get run over and the pine trees they are under were not removed.  I was able to exit on to the road avoiding the deep ditch hiding in the long grass.

[attachimg=1]

[attachimg=2]

[attachimg=3]

I also spotted this lone Dactylorhyza maculata

[attachimg=4] 
Title: Re: Terrestrial Orchids 2019
Post by: Yann on August 24, 2019, 06:19:18 PM
excellent to see this species so close to your house :o
Title: Re: Terrestrial Orchids 2019
Post by: Gabriela on August 31, 2019, 01:23:50 AM
Yes, nice indeed to have so many Goodyera close by. They are flowering early, by Canadian standards :)

One orchid from me; quite common in some areas and not a stranger to Europe as well, but to show it growing in alvars habitat - Spiranthes romanzoffiana.
[attachimg=1]
Title: Re: Terrestrial Orchids 2019
Post by: ebbie on October 16, 2019, 08:42:35 AM
Spiranthes cernua, a late-blooming bog orchid.
(https://up.picr.de/37003782rk.jpg)
Title: Re: Terrestrial Orchids 2019
Post by: Anders on October 18, 2019, 09:57:13 AM
Roma, nice G. repens colony. It was found the first time in Denmark in 1878, when there was almost no forest in Denmark. It has become more common since then in old pine plantations, but it is still rare. I germinated G. repens seeds about 10 years ago, they are very easy to propagate in vitro. They flowered once in the garden, and then the the slugs ate all of them. Thanks to Gabriela, I now have G. oblongifolia  in my flasks. I plan to keep some in the greenhouse, where the slugs can be controlled. S . cernua is also easy in vitro. I deflasked them four years ago, and they have flowered in the garden every year as the last orchids , I took the photo today.
Title: Re: Terrestrial Orchids 2019
Post by: brianw on December 26, 2019, 07:48:09 PM
Calanthe.
Like Gail earlier this year I was seduced by some garden center pots; especially as they were £20 reduced to £5 in the winter sale ;-) My plants (3) all have 4 or 5 side growths and passable but tired, green leaves. Almost all side growths have new shoots at soil level. Any recommendations in what to do when. Compost I can deal with but division or pot on, and when I am new to. They will be grown under glass, frost free or not, for the time being at least. Thank you.
Not often I have a GC till receipt that says £25 with £52 discount ;-)
Title: Re: Terrestrial Orchids 2019
Post by: Gail on December 30, 2019, 07:31:39 AM
Calanthe.
Like Gail earlier this year I was seduced by some garden center pots; especially as they were £20 reduced to £5 in the winter sale ;-) My plants (3) all have 4 or 5 side growths and passable but tired, green leaves. Almost all side growths have new shoots at soil level. Any recommendations in what to do when. Compost I can deal with but division or pot on, and when I am new to. They will be grown under glass, frost free or not, for the time being at least. Thank you.
Not often I have a GC till receipt that says £25 with £52 discount ;-)
Sounds a bargain!
The potful I showed earlier I wasn't sure what to do with so I took a knife to it and divided into 5 pieces, while in full flower...
Somewhat to my astonishment all survived and are still in leaf (as you say passable but tired, green leaves). One division was given to a neighbour, one planted in the bed I pictured and the others remain in pots and have new shoots. I hope they are winter hardy because it seems a very good plant!
Title: Re: Terrestrial Orchids 2019
Post by: Jan Jeddeloh on January 02, 2020, 02:51:43 AM
Anders,
Those are some impressive Goodyera orchids you have there.  What medium did you use?  I've been to your website http://www.invitroorchids.dk/en_index.html (http://www.invitroorchids.dk/en_index.html) and looked at the media recipe you list.  Is this what's been successful for all your orchid seed sowing ventures?  Anything it's not successful on? I've also been growing orchids from seed but I've been using specialized media for some.

Here's what I've used:
Bletilla- 1/2 MS salts, 20 gr sugar
Dactylorhiza- Malmgren Modified Terrestrial Orchid media (Phytotech Labs), 20 gr sugar
Pleione-Orchid seed sowing media P668 (Phytotech Labs), 20 gr sugar
Gelzan 2-3 gr per liter
Ph adjusted to 5.6-6.2

Based on your results it looks like dacts should do fine on the 1/2  MS salts. I have some fresh pleione seed (Golden Gate x Tonogiro) that I'll try on the same media.  I'd like to avoid specialized media if I can.  Following the KISS (keep it simple stupid) principle is a good idea in most ventures. 

My most recent seed sowing ventures involved dry seed because I got lazy and didn't harvest the pods in time.  I must say I strongly prefer the green pod method.  It's a lot less work.

Have you ever considered building a simple transfer box so you don't have to work over a steaming kettle?  I have a homemade one I sterilize with alcohol before use and then use alcohol and an alcohol burner (Bunsen burner) to sterilize the tools.  I also do plant tissue culture on lilies and trillium and this set up works great so that too.  Considering we (my friend and I ) don't have a HEPA filter we really don't have much contamination. 

I'd love to hear more about all the orchids you've germinated.

Jan Jeddeloh, Portland, Oregon, USA  More or less zone 8
Title: Re: Terrestrial Orchids 2019
Post by: Anders on January 05, 2020, 01:26:42 PM
Hi Jan

Yes, I use the simple liquid fertiliser medium for all  terrestrial orchids, but slightly more concentrated (3 mL/L) than described on the homepage. Yes, I know, I haven't updated my homepage for years, but this spring....(hopefully). My medium is as simple as possible and only have ingredients that you can buy at any supermarket or from Amazon. I started out with a more complex recipe, but have gradually made it more simple as there seems to be little or no effect of compounds such as myo-inositol, adenine, peptone, and pure lab-grade mineral nutrients vs. a general liquid fertiliser for pot plants. If the protocorms show browning on the fertiliser medium, I sometimes also use a glycine medium with inorganic nitrogen inspired by Malmgren's media. I think that any general plant tissue medium could be used as long as it is not too concentrated, 1/2 MS is probably in the upper end, I would suggest 1/3 MS or less. Agar quality is important, I use the brand SpecialIngredients obtained from amazon.co.uk. Food agar from Asian shops is sometimes low-quality and toxic for the protocorms. If you use professional agar substitutes such as Gelzan (gellan gum), you don't have to worry about quality, but Gelzan requires a rather high concentration of magnesium and/or calcium to solidify and probably wouldn't work with my recipes unless you add 4-5 g/L.

And yes, I have a sterile transfer box, actually, I haven't used the steaming frying pan method for more than 15 years. I have written a short instruction on how to build a very cheap HEPA-filter transfer box (sterile cabinet) based on the same principles as the professional version.
http://invitroorchids.dk/pdf/7sterilecabinet.pdf (http://invitroorchids.dk/pdf/7sterilecabinet.pdf)

At the moment, I have flasks with various Cypripedium, Pleione, Dactylorhiza, Bletilla, Anacamptis, Serapias Goodyera and Thelymitras (thanks to Steve G for many of the seeds). I have only had limited success with American Plantanthera, but that is probably mostly a question of using the green pod method instead of mature seeds, the problem is that I don't have a source of green pods or fresh seeds. Epipactis is also impossible from mature seeds, and only a few seeds have germinated from green pods. And then there is Dactylorhiza sambucina. They germinate well, but then turn brown and die. I have tried many different seed batches with the same poor result.

Do you have a homepage about your orchids, lilies and trillium?

Anders

Fertlizer medium, per litre:
Liquid mineral fertilizer for indoor plants    3 ml
Potato                                                    25 g
Fresh orange juice                                  25 ml
Sucrose (table sugar)                              12 g
Agar                                                        6 g
pH                                                          6.8sorry, should be  5.8

Glycine medium, per litre:
CaCl2 - 2H20                50 mg
K2HPO4                      100 mg
MgSO4 - 7H2O              50 mg
FeSO4 - 7H2O               20 mg
MnSO4 - 1H2O              10 mg
Glycine                       700 mg
Potato (blended)           25 g
Fresh orange juice        25 ml
Sucrose (table sugar)   12 g
Agar                             6 g
pH                               6.8
Title: Re: Terrestrial Orchids 2019
Post by: Anders on January 06, 2020, 07:29:47 AM
Oh no! Wrong pH - should have been 5.8, NOT 6.8. Sorry.

Anders
Title: Re: Terrestrial Orchids 2019
Post by: Jan Jeddeloh on January 14, 2020, 03:56:26 AM
That's a pretty fancy transfer box you have there Anders.  How do you sterilize your tools?  I don't see a Bunsen burner in your equipment.  I'd like to get away from using one but the bead sterilizers are really expensive.  Like you I'm into cheap and easy.

Have you ever tried sowing seeds on media that did not contain potato and orange juice?  I wonder if they're absolutely necessary.  Again looking for the simplest way possible. 

I was going thru my seed stash last night and I have all kind of dactylorhize and bletilla seed to try.  The weather is cold and nasty outside so this will give me something to do inside.  Some of my seed is quite old but it's been stored in the fridge so could still be viable.  I also have some plantathera and epipactis seed.  Sounds like I might as well pitch the epipactis seed based on your experience. 

No I don't have a website.  I've thought about starting one but it seems like it's a lot of work to keep one up.

Jan

Title: Re: Terrestrial Orchids 2019
Post by: Neil on January 14, 2020, 10:34:23 AM
Jan I sterilise all my tools in a pressure cooker 15psi for 20 minutes, before transferring them to the clean area.
Title: Re: Terrestrial Orchids 2019
Post by: Anders on January 15, 2020, 09:11:45 PM
Jan, I sterilise my tools by dipping in ethanol and the setting them om fire with a lighter. Very simple and standard procedure in microbiology labs. I do this every time I open a new flask to avoid crosscontamination. I flame them inside my transfer box, but you might also do it outside the box right before use. You can see the ethanol beaker to the right in the transfer box (actually a slender olive jar placed in a jam jar so it doesn't tip over - you don't want burning ethanol all over the transfer box!!).

Potato and orange juice is a simple way of adding hormones, vitamins, trace elements and buffer capacity. I am sure you could germinate many species on a purely inorganic medium like 1/2MS or Knudson C. I haven't tried myself, but there are many examples in the scientific literature. 

Anders
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